QuestionsHow to growTrees and ShrubsWeedsRoundup 0 Vote Up Vote Down oliver sexton asked 13 years ago Whats the difference between Round up and Round up pro Biactive. which is best as a general weedkiller? 1 Answers 0 Vote Up Vote Down Gerry Daly Staff answered 6 years ago Roundup and Roundup Pro Biactive both contain the same amounts of active ingredient glyphosate. But the Biactive formulation has added surfactants that allow better entry of the herbicide into the weed foliage.